Scientists trace origin of our teeth from most primitive jawed fish

According to an International team of scientists, the origin of our teeth goes back more than 400 million years back in time, to the period when strange armoured fish first developed jaws and began to catch live prey.

We are the descendants of these fish, as are all the other 60,000 living species of jawed vertebrates - sharks, bony fish, amphibians, reptiles, birds, and mammals.

The team of scientists led by Uppsala University (Sweden), in collaboration with the ESRF, the European Synchrotron (France), the brightest X-ray source, has digitally 'dissected', for the first time, the most primitive jawed fish fossils with teeth found near Prague more than 100 years ago.

The results, published in Science, show that their teeth have surprisingly modern features.

Teeth in current jawed vertebrates reveal some consistent patterns: for example, new teeth usually develop on the inner side of the old ones and then move outwards to replace them. There are, however, several differences between bony fish (and their descendants the land mals) and sharks; for example the fact that sharks have no bones at all, their skeleton is made of cartilage, and neither the dentine scales nor the true teeth in the mouth attach to it; they simply sit in the skin.

In bony fish and land mals, the teeth are always attached to jawbones. In addition, whilst sharks shed their worn-out teeth entire, simply by detaching them from the skin, bony fish and land mals shed theirs by dissolving away the tooth bases.

A team from Uppsala University, Charles University (Czech Republic), Natural History Museum in London (UK), National Museum in Prague (Czech Republic) and the ESRF, the European Synchrotron (France) set out to determine whether this particular type of dentition was really ancestral to ours, or just a specialised offshoot off the lineage leading towards modern jawed vertebrates.

With this aim, they turned to the acanthothoracids, another early fish group that are believed to be more primitive than the arthrodires and closely related to the very first jawed vertebrates. The problem with acanthothoracids is that their fossils are rare and always incomplete.

The very finest of them come from the Prague Basin in the Czech Republic, from rocks that are just over 400 million years old, and were collected at the turn of the last century. They have proved difficult to study by conventional techniques because the bones cannot be freed from the enclosing rock, and have therefore never been investigated in detail.

The researchers used the unique properties of the ESRF, the world's brightest X-ray source and the synchrotron microtomography ID19's beamline, to visualise the internal structure of the fossils in 3D without damaging them. At the ESRF, an 844 metre-ring of electrons travelling at the speed of light emits high-powered X-ray beams that can be used to non-destructively scan matter, including fossils.

"The results were truly remarkable, including well-preserved dentitions that nobody expected to be there" said Valeria Vasknova, lead author of the study and scientist from Uppsala University. Follow-up scans at higher resolution allowed the researchers to visualize the growth pattern and even the perfectly preserved cell spaces inside the dentine of these ancient teeth.

Like arthrodires, the acanthothoracid dentitions are attached to bones. This indicates that bony fish and land mals retain the ancestral condition in this regard, whereas sharks are specialized in having teeth that are only attached to the skin - in contrast to the common perception that sharks are primitive living vertebrates. Again, like arthrodires, the teeth of acanthothoracids were not shed.

In other ways, however, acanthothoracid dentitions are fundamentally different from those of arthrodires. Like sharks, bony fish and land mals, acanthothoracids only added new teeth on the inside; the oldest teeth were located right at the jaw margin. In this respect, the acanthothoracid dentitions look remarkably modern.

"To our surprise, the teeth perfectly matched our expectations of a common ancestral dentition for cartilaginous and bony vertebrates." explained Vasknova.

The tooth-bearing bones also carry small non-biting dentine elements of the skin on their outer surfaces, a character shared with primitive bony fish but not with arthrodires. This is an important difference because it shows that acanthothoracid jaw bones were located right at the edge of the mouth, whereas arthrodire jaw bones lay further in.

Uniquely, one acanthothoracid (Kosoraspis) shows a gradual shape transition from these dentine elements to the neighboring true teeth, while another (Radotina) has true teeth almost identical to its skin dentine elements in shape. This may be evidence that the true teeth had only recently evolved from dentine elements on the skin.

"These findings change our whole understanding of the origin of teeth" said co-author Per Ahlberg, professor at Uppsala University.

And he adds: "Even though acanthothoracids are among the most primitive of all jawed vertebrates, their teeth are in some ways far more like modern ones than arthrodire dentitions. Their jawbones resemble those of bony fish and seem to be directly ancestral to our own. When you grin at the bathroom mirror in the morning, the teeth that grin back at you can trace their origins right back to the first jawed vertebrates."

( With inputs from ANI )